Introduction: Submerged vanes are flow-pattern altering structures that are mounted vertically on channel-bed at a small angle of attack to the approach flow. A submerged vane generates a secondary circulation (a spiral flow), due to the vertical pressure gradients on the two sides of the vane, which originates below the top elevation of the vane and extends in the downstream of the vane. The vane-induced vortex redistributes sediment within the channel cross section and changes the alluvial bed profile. However local scour around the vanes is one of the problems in using of submerged vane technique. The extension of local scour hole is related to the shape of the vanes. Primary submerged vanes are generally flat rectangular plates. In the present research, cutting a part of the leading edge of the vanes out is studied as a countermeasure in reducing the local scour. Studied vanes include a rectangular vane (as the baseline vane), and five other modified vanes with tapered leading edges with angle of  = 30° , 45° , 60° , 70° , and 73. 3° . The present study aims to evaluate the effect of this modification on the vertical velocity components at the leading edge and strength of the secondary circulation in the downstream of the vanes. Flow-3D numerical model, version 10, is used to study the flow field around the vanes. Methodology: The commercial CFD model Flow-3D was used in this research. Experimental velocity measurements were used for calibration of the model. For this purpose, a recirculating flume (7. 30 m long by 0. 56 m wide by 0. 6 m deep) was used. A centrifugal pump discharged the water into the stilling tank at the entrance of the flume. In order to create a uniform inflow of water, a screen was placed at a distance of 1 m from the flume entrance. A tail gate was used to adjust the depth (do) of water in the flume to a constant value of 0. 25 m. The dimensions of the vanes were determined using Odgaard’ s (2008) design criteria: a vane height-to-water depth ratio of Ho/do = 0. 3 and length of L = 3Ho. A mean flow depth of do = 0. 25 m yielded Ho = 0. 075 m and L = 0. 25 m. velocity measurements carried out using vanes V0 and V3 at a flow Froude number of Fr = 0. 16. In each test, the vanes were installed on the centerline of the flume at an angle of 20° to the flow. In order to study vane-induced velocity field, 4×4 cm 2 grids across the flume were taken at the center of the vanes. At each grid point, three-dimensional components of velocity vector (u, v, w) were measured by means of an electromagnetic velocimeter (EVM). Velocity very close to the walls of the flume was not measured. Results and discussion: On the high-pressure side of the vanes, vertical velocity components were upward (positive) and on the low-pressure side were downward (negative). Therefore, a clockwise secondary circulation was generated at downstream of the vanes. Downward velocity components at leading edge of primary rectangular vane (vane V0) were obvious. By cutting parts of leading edge out of vane V0 for tapered vanes V1 and V2, the magnitude of negative w-velocity components was respectively reduced by 40% and 69%. By increasing the taper angle for vanes V3, V4 and V5, downward velocity components were diminished, effectively. Moment of momentum (MOM) quantity was used in order to evaluate strength of vaneinduced circulation. MOM values were applied for comparison of performance of the vanes. For this purpose, velocity data at two sections at the distances of 2Ho and 4Ho, i. e., 15 cm and 30 cm downstream from center of the vanes was used. In the calculation of MOM, 100 velocity components (50 v-components and 50 w-components) were used. Therefore, this quantity is a useful criterion for evaluation of the performance and efficiency of the submerged vanes. Conclusion: Velocity distribution and moment of momentum (MOM) of the vanes indicated the reduction of erosive negative velocity components at the leading edge of the tapered vanes. Based on MOM values, cutting the leading edge out of the vanes causes lower performance. In other words, this modification restricts the vane-influenced field of the tapered vanes relative to the rectangular vane (vane V0). Results showed that the performance of tapered vanes (V1 to V5), relative to the rectangular vane, (at distance of 2Ho) is respectively reduced by 5. 8%, 7. 3%, 17. 8%, 33% and 42. 6%; at distance of 4Ho the amount of reduction respectively is 7. 4%, 11. 9%, 17%, 25. 5% and 34. 3%. On the contrary, the efficiency of the tapered vanes increased. The amount of increasing at distance of 2Ho from the center of vanes V1 to V5 respectively is 3. 2%, 9%, 11%, 14% and 14. 8% and at distance of 4Ho respectively is 1. 4%, 3. 6%, 12. 1%, 26. 7% and 31. 3%. Therefore, if tapered vanes are used to reduce the local scour, big values for the distance between the vanes arrays (δ s), according to the design criteria, are not recommended.

Study of separation zone is so important in right-angled three-branch or four-branch open channel junctions. Some effective parameters in this case are inlet discharge ratio and flow depth which in this research the effect of inlet discharge ratio and weir height ratios (flow depth) on flow pattern and dimensions of separation zone has been simulated numerically. Investigation of numerical results showed that k-ω model well validated with experimental results and had good agreement, so that simulation error was less than 20%. Dimensions of separation zone in main and side channels were directly proportional with the inlet discharge ratio. Also as increases of height ratio of outlet weirs, flow depth increases and separation zone dimensions decreased. According to the analysis of numerical results, dimensions of separation zone in vertical direction, of the channel bed to water surface increased, so that for discharge ratio 0.6 and height ratio of outlet weirs 0.377, length of separation zone at the channel bed, 0.1 m up the bed and water surface was about 60 cm, 75 cm and 85 cm, respectively. So of the water surface towards the channel bed, length of separation zone decreased about %29.

Foot and Mouth Disease (FMD) vaccine is one of the inactivated vaccines and its potency depends on the antigen quality, antigen concentration per dose, and adjuvant. In this study to introduce the better antigen concentration method, both active and inactive of three serotypes O, A05, and Asia of FMD virus were used. Each of the virus type was cultivated in BHK21 cell culture and half of each viral suspension inactivated by ethylene imine. The physical method; ultra filtration (cross flow system) and the chemical method; treatment with poly ethylen glycole (PEG6000) were used for virus concentration following safety assay. The quantity of virus recovery was evaluated by virus adsorption by aluminum hydroxide gel, complement fixation, ELISA, TCID50 titration, and sucrose concentration on the collected samples. The results indicate that the concentration rate did not affect virus adoption by aluminum hydroxide gel and the quantity of virus recovery using ultra filtration was higher than PEG precipitation.

Bottom intake is an intake structure which can be considered as an alternative for regular intakes in mountain streams Water in in taking from river are very vast and complicated issues, each project has special characteristics which should be considered. Water intake from river is generally based on Pump and gravity method. The gravity method is more useful and common because of continuity and no need spare energy rather than pump method. The different methods are possible at gravity methods these methods are divided in to three categorize: side in take, front structure and bottom structure.According to the recent experiences at countries for maintain region, when longitude slope is more than 1% and 75% of bed load is particles with size more than of mm (D50>6mm) bottom in take are recommended generally. Hydraulic analyses of this type of structure are not studied numerically. In other side of Cain, building and studying physical model of this type of structure are very difficult tasks. In this research, flow simulations of bottom in take are done using the flow 3D software. As a result, it's conducted that best Slope for intake net is degree and when opening area. Increased directed discharge increase directly. Finally it's driven that when Froude number increased opening percentage, it May caused reduction of flow coefficient.